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Samad, Abdul [1].

A Protocol for Nonradioactive Differential Display Optimized for Cotton (Gossypium hirsutum L.) Fiber RNA.

A protocol for nonradioactive differential display visualized by ethidium bromide staining was optimized for cotton (Gossypium hirsutum L.) fiber total RNA. The procedure was used to identify genes differentially expressed at 0 DAP (Day After Pollination) and 10 DAP of developing cotton fiber. Different populations of total RNA were reversely transcribed using three single anchored oligo-dT primers. PCR amplification of relative cDNA was carried out in combination with five arbitrary primers (random decamers). New one percent agarose containing ethidium bromide for staining was a simplified protocol that can resolve amplified transcripts over a large range of molecular weights. Several differentially expressed transcripts were successfully identified by this procedure.


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1 - Quaid-i-Azam University, Department of Biological Sciences, Islamabad, 68000, Pakistan

Keywords:
Nonradioactive
Differential Display.

Presentation Type: Array
Session: 33-136
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM
Abstract ID:678


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