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Recent Topics Posters

Peñaflor, Cynthia [1], Boore, Jeffrey L. [2], Kuehl, Jennifer V. [3], Jansen, Robert K. [4].

Chloroplast Genome Sequences of Chloranthus and Piper: Implications for the Phylogeny of a Angionsperms.

We have sequenced the chloroplast genomes of two members of the Eugmagnoliids, Chloranthus (Chloranthales) and Piper (Piperales), The Chloranthus genome is 98% complete and the Piper genome is complete. Both genomes have the identical gene order and gene content as tobacco, which has the typical genome organization for angiosperms. The Piper genome is 160,625 bp long with a inverted repeat of 27,038 bp. small single copy region of 18,880 bp and a large single copy region of 87,669 bp. We extracted 61 protein coding genes from both genomes and aligned these sequences to dataset of 25 other genomes representing many of the major lineages of angiosperms and several outgroups from other land plant taxa. Phylogenetic analyses were performed on a concatenated dataset including all 61 genes using both parsimony and maximum-likelihood methods. Both methods of phylogenetic analysis place Amborella and the Nymphaeales in a basal position and Chloranthus and Piper in a clade with the Eumagnoliid Calycanthus near the base of the angiosperm tree. This result is in agreement with recent phylogenies based on several chloroplast, mitochondrial, and nuclear genes.


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1 - Brigham Young University, Integrative Biology, Provo, UT, 84604, USA
2 - DOE Joint Genome Institute, Walnut Creek, CA, , USA
3 - DOE Joint Genome Institute, Department of Evolutionary Genomics, 2800 Mitchell Drive, Walnut Creek, California, 94598, USA
4 - Universtiy of Texas Austin, Section of Integrative Biology, 1 University Station, A6720, Austin, Texas, 78712, USA

Keywords:
Angiosperm Phylogenetic Relationships
chloroplast genomics.

Presentation Type: Recent Topics Poster
Session: 33-129
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM
Abstract ID:671


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