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Systematics Section / ASPT

Wada, Eric [1], Walker, M. Andrew [1], Potter, Daniel [2].

Phylogeny of Vitis Based on cpDNA and nrDNA Sequences.

The taxonomy of wild species of Vitis has been reputed to be difficult. Classification schemes differ in the number of recognized species within the genus. The incidence of natural hybridization in the wild is thought to confuse Vitis taxonomy, however no molecular studies verifying that hybridization occurs have been conducted in Vitis. The purpose of our study was to reconstruct phylogenetic relationships within Vitis in an attempt to describe species boundaries. Plants in the study representing most of the species within Vitis were acquired from the UC Davis Viticulture and Enology vineyards and the USDA germplasm repository in Davis, CA. We also included several individuals recently collected from wild populations in Texas, New Mexico, and Arizona. The Internal Transcribed Spacer (ITS) region of nuclear ribosomal DNA, and an intergenic spacer of the chloroplast genome were sequenced. Preliminary analysis suggests that speciation is a relatively recent event in Vitis. Species currently distributed throughout the southwestern United States exhibit low sequence variation; some currently recognized species might actually represent hybrids of other species. The ITS region is potentially useful for identifying recent hybrids in the wild. Finally, the use of microsatellite markers to characterize the incidence and frequency of hybridization within Vitis will also be discussed.


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1 - University of California Davis, Department of Viticulture & Enology, One Shields Avenue, Davis, California, 95616, USA
2 - University of California Davis, Section of Plant Biology, One Shields Avenue, Davis, California, 95616-8537, USA

Keywords:
Vitis
Phylogeny
hybridization.

Presentation Type: Oral Paper
Session: 12-3
Location: 410/Hilton
Date: Monday, August 15th, 2005
Time: 1:15 PM
Abstract ID:382


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