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Systematics Section / ASPT

Murfett, Jane [1], Lee, Chris [1], Page, Larry [1], Yates, Allena [1], Ippolito, Anthony [2], Holtsford, Timothy P. [1].

Phylogenetics of Nicotiana Section Alatae and description of N. rastroensis.

We studied species relationships in Nicotiana Section Alatae with nrITS sequences, 256 ISSR bands, interspecific crossability, floral morphology and corolla and calyx development. Nuclear ribosomal internal transcribed spacer (nrITS) DNA sequences supported a monophyletic Alatae, but one that does not include N. sylvestris (Ippolito 2000, Chase et al. 2003, cf. Goodspeed 1954). Within Alatae one clade was resolved consisting of the n=10 species, N. longiflora and N. plumbaginfolia. Ippolito's nrITS tree also suggested a paraphyletic relationship of N. plumbaginifoia to N. longiflora. This was confirmed using 256 ISSR bands in an Alatae-wide parsimony tree and further resolved in a tree containing 14 populations and 24 individuals of the n=10 species.  We have observed 3 populations in NW Argentina where N. longiflora and N. plumbaginifolia are sympatric and putative hybrids were seen. However, most the of the intermediate morphs form a highly supported clade suggesting the medium floral morphs may represent a separate evolution of smaller flowers. The Alatae-wide ISSR tree also found a weakly supported clade containing N. bonariensis, as sister to the rest of the n=9 group. Crossability interpretations are complicated. Style length was a very good predictor of interspecific crossing success as measured by seed set and pollen tube growth rates. This suggests that Biological Species Concept boundaries could evolve as a pleiotropic result of flower size evolution, or vice versa. Floral organ dimensions and developmental trajectories suggest the following pairs of species are most closely related to each other: N. mutabilis and N. langsdorffiii; N. rastroensis and N. bonariensis; N. alata and N. forgetiana (the last hypothesis is also suggested by biogeography), but these relationships were not always supported by crossability. Crossability, morphology, development of flowers and daily phenology suggest a species be erected for N. rastroensis.


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1 - University of Missouri-Columbia, Biological Sciences, 105 Tucker Hall, Columbia, Missouri, 65211, USA
2 - De Paul University, Department of Biological Sciences, 111 MacGowan Hall, Chicago, Illinois, 60614, USA

Keywords:
Nicotiana
Solanaceae
systematics.

Presentation Type: Poster
Session: 33-97
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM
Abstract ID:359


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