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Developmental and Structural Section

Bubu, Oghenerhoro [1], Fuller, Mick [2].

Production, storability, dehydration and regeneration of micro-shoots of cauliflower encapsulated in soduim alginate beads.

Micro-shoots of cauliflower were produced from fractionated and graded curds of Medaillon and an unknown variety and encapsulated in 3% sodium alginate after 2 weeks culture in liquid MS medium. Micro-shoots of both varieties achieved 80% conversion into plantlets and encapsulation did not reduce their viability. Artificial seed were stored at 4°C for 28d without decline in viability. Cryopreservation treatments of –20°C, -80°C, or placed into liquid Nitrogen and then –80°C for 1 week, even after 28d acclimation at 4°C killed the micro-shoots as no generation occurred. Dehydration of fully hydrated seeds from 96% to 67%, 35% or 16% moisture content had no detrimental effect on their conversion into plantlets with conversion rates of 68-78% been recorded. These results clearly demonstrate that artificial seeds of cauliflower can be stored successfully at 4°C for a considerable time and can also be dehydrated to moisture content similar to true seed without loss of viability.


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Somatic embryogenesis and synthetic seeds


1 - 3 Mayfield Crescent, Bradley Barton,, Newton Abbot, TQ12 1PF, United Kingdom
2 - University of Plymouth, Head School of Biological Sciences, University of Plymouth, Drake Circus, Plymouth, Devon, PL4 8AA, United Kingdom

Keywords:

Microshoots
Artificial seeds
Cauliflower
Viability.

Presentation Type: Oral Paper
Session: 31-5
Location: Salon A - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 11:15 AM
Abstract ID:312


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