Peters, Melinda , Xiang, Jenny Qiu-Yun , Stucky, Jon , Vargo, Edward .
Genetic diversity and outcrossing rate in the federally endangered Echinacea laevigata (Asteraceae) - Inferred from amplified fragment length polymorphism (AFLP).
Echinacea laevigata is a federally endangered species and a close relative of the medicinally important E. purpurea. The species has 24 recognized populations restricted to four states (VA, NC, SC, GA). To determine the population structure and outcrossing rate across the range of the species, we conducted AFLP analysis using four primer combinations for 22 populations, each including approximately 20 individuals. Preliminary results from one primer combination for 12 populations including 216 individuals show a level of gene diversity ranging from 0.1497-0.3094 (mean = 0.2098) and a GST of 0.262. Sixty-six out of the 71 loci (93%) are polymorphic. These results suggest relatively high genetic diversity in the species and there is substantial genetic differentiation among the populations, congruent to a previous allozyme study. A preliminary estimate of the outcrossing rate based on genotypes of mother and progenies from the largest population and 212 loci from all four primer combinations is 0.989, indicating that the population is predominantly outcrossing. Analyses of data from other populations are in progress. The results will be presented and discussed along with their implications in population structure and conservation.
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1 - North Carolina State University, Department of Botany, Campus Box 7612, Raleigh, North Carolina, 27695-7612, USA
2 - North Carolina State University, Department of Entomology, Campus Box 7613, Raleigh, North Carolina, 27695-7613, USA
Presentation Type: Oral Paper
Date: Monday, August 15th, 2005
Time: 11:45 AM