Roper, Jessie M. , Hansen, Sterling K. , Murdock, Andrew G. , Kuehl, Jennifer V. , Boore, Jeffrey L. , Karol, Kenneth G. , Mandoli, Dina F. , Olmstead, Richard , Mishler, Brent D. , Wolf, Paul G. .
Chloroplast genome sequence of the marattioid fern Angiopteris.
We isolated chloroplasts from cell extracts of Angiopteris evecta using Fluorescence Activated Cell Sorting (FACS). DNA from these chloroplasts was amplified via rolling circle amplification. The genome was then subcloned and shotgun sequenced to 8X coverage. Long PCR was used to bridge gaps and complete assembly of the circular genome sequence. We annotated the sequence and compared the Angiopteris chloroplast genome to that of other land plants. Gene order in Angiopteris is similar to that of the whisk fern Psilotum with a few notable exceptions. Relative to Psilotum, the rpl21, rpl32, ndhf, and several trn genes have moved from the inverted repeat region to the small single copy region. Angiopteris is hypothesized to be part of a lineage (marattioid ferns) that emerges near the base of the monilophytes, and possibly as a sister to horsetails. We show how information from gene order, sequence length variation, alignment of nucleotide sequences, and morphological data can be used together to reveal aspects of the phylogenetic position of Angiopteris and shed light on relationships at the base of vascular plants.
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1 - Utah State University, Biology, 5305 Old Main Hill, Logan, Utah, 84322, USA
2 - DOE Joint Genome Institute, Department of Evolutionary Genomics, 2800 Mitchell Drive, Walnut Creek, California, 94598, USA
3 - University of Washington, Department of Biology, Po Box 35-1800, Seattle, Washington, 98195-1800, USA
4 - University of California, Berkeley, Department of Integrative Biology, 1001 Valley Life Sciences Bldg. #2465, Berkeley, California, 94720-2465, USA
Presentation Type: Poster
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM