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Genetics Section

Kolar, Elizabeth A. [1], Wiley, Michael [1], Burnett, Alison L. [1], Parke, Krispen [1], Hunter, Richard [1], Hunter, Kimberly [1].

Development of microsatellite markers in Larrea (Zygophyllaceae): A new way to investigate the evolutionary history of Larrea.

Larrea is one of the dominant perennials in the deserts of North and South America. North American Larrea tridentata has three ploidy levels in three distinct regions: Chihuahuan Desert - diploid; Sonoran Desert- tetraploid; Mojave Desert- hexaploid. In South America there are four species: L. divaricata, L. cuneifolia, L. nitida, and L. ameghinoi. A variety of molecular techniques (ITS, cpDNA, ISSRs) have been utilized to determine the phylogeny of this small genus, and to differentiate between the ploidy levels of L. tridentata. However, all of these methods did not yield sufficient levels of variation. Microsatellites have been shown to be useful in population genetic studies, and because of their hypervariability offer a method for investigating important evolutionary relationships in Larrea. Using an enrichment protocol, a microsatellite library has been developed for Larrea. The results obtained will be important for understanding the distribution of Larrea polyploids in North America and for determining the phylogeny of the genus.


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1 - Salisbury University, Department of Biology, 1101 Camden Avenue, Salisbury, Maryland, 21801, USA

Keywords:
microsatellites
Larrea
polyploidy
Zygophyllaceae
desert.

Presentation Type: Poster
Session: 33-44
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM
Abstract ID:294


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