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Genetics Section

Liu, Xianan [1], Swire-Clark, Ginger [2], Baird, Wm. Vance [2].

A cDNA encoding a blight-associated protein-like gene from sunflower is up-regulated by high salinity, drought and exogenous ABA.

The blight-associated protein p12, whose sequence is similar to that of expansin, has been characterized from only a few species and its function is still unknown. The expression of the p12 gene in response to stimuli other than pathogen attack was investigated. A partial cDNA (RSC1-U), originally found to be up-regulated by high salinity, was cloned from seedling roots of sunflower (Helianthus annuus) by differential display reverse transcriptase-PCR. The full-length cDNA was then cloned by rapid amplification of cDNA ends. This cDNA is 578 bp in length, with a 390 bp ORF coding for 130 amino acid residues. The deduced amino acid sequence was found to have 52% identities and 66% similarities to the blight-associated protein p12 of citrus. The sunflower blight-associated protein-like gene, designated HaBAP12, was constitutively expressed at a low level in plant shoots and at a very high level in leaves, but not expressed in seedling roots. The expression of HaBAP12 in response to abiotic/environmental stress and exogenous abscisic acid was also analyzed. The expression of HaBAP12 was in an organ-specific manner, and up-regulated in both seedling roots and shoots by exposure to high salinity, drought and exogenous application of abscisic acid. This suggests that HaBAP12 may be involved in the general stress response of plants to stress conditions, both biotic and abiotic.


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1 - Colorado State University, Department of Biology, 1878 Compus Delivery, Fort Collins, Colorado, 80521, USA
2 - Clemson University, Horticulture Department, Poole Agr Cntr, Box 340319, Clemson, South Carolina, 29634-0319, USA

Keywords:
abiotic stress
Asteraceae
blight-associated protein
environmental stress
Gene expression
sunflower.

Presentation Type: Poster
Session: 33-55
Location: Salon C, D & E - Gov Ballroom/Hilton
Date: Tuesday, August 16th, 2005
Time: 12:30 PM
Abstract ID:184


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